Molekuler Filogenik Burung Weris (Galliralus Torquatus) menggunakan Gen CO1 DNA Mitokondria

Orlendy Gasah, Mecky Pandelaki, Indra Rompas


The zebra mandar bird (Galliralus torquatus) or in the local language (Minahasa) is known as the weris bird while in Kotamobagu and Bolmong the boruit is known as a wild bird species, and is a bird species whose distribution is not so wide with a relatively small population and breeding slow. Weris, which is one of the characteristics of the Sulawesi region, needs conservation to maintain this biological asset. One of the forms is to maintain the diversity of the genetic level of this species in the form of molecular data collection by studying its phylogenetic molecular through DNA barcodes. With molecular phylogeny, the evolutionary history and kinship relationships of weris with nearby species can be known and conservation and data collection of species diversity at the genetic level can be carried out and obtained. Based on this, this research will conduct a molecular study of the phylogenetic of weris birds using the standard COI gene (Cytochrome oxidase sub unit 1). the results of the sequence analysis obtained the length of the COI gene in both samples of weris, namely WRS 1 with 661 bp and WRS 2 with 652 bp and the phylogenetic tree construction showed that weris had the closest kinship with the Gallirallus okinawae species. Weris WRS1 and WRS2 tend to form new clades leading to the formation of new species

Keywords: Phylogenic Molecular, CO1 Gene, Zebra Mandar bird

Teks Lengkap:

Tidak berjudul


Consortium for the Barcoding of Life (CBoL) Plant Working Group. 2009. A DNA barcode for land plant. Proceedings of the National Academy of Sciences USA, 106: 12794-12797.

Coast B. J, Bishop KD. 2000. Panduan Lapangan Burung-Burung di Kawasan Wallacea. (Sulawesi, Maluku, dan Nusa Tenggara). BirdLife International.

Desjardins, P. and R. Morais. 1990. Sequence and gene organization of the chicken mitochondrial genome. A Novel Gene Order in Higher Vertebrates. J. Biol. (212): 599-634

Desjandris, P. and R. Morais. 1991. Nucleotide sequence and evolution of coding and non coding regions of a quail mitochondrial genome.J. mol. Evo/.(32):153161

Drummond AJ, Ashton B, Buxton S, Cheung M, Cooper A, Duran C, Field M, Heled J, Kearse M, Markowitz S, Moir R, Stones-Havas S, Sturrock S, Thierer T, Wilson A (2012) Geneious v5.6. Biomatters. New Zealand

Edgar RC (2004) MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acid Res 32 (5): 1792-1797

Frezal L, Leblois R (2008) four years of DNA barcoding: current advances and prospects. Infect genet Evol 8:727-736

Fumihito, A, T. Miyake, S. Sumi, M. Takada, S. Ohno, and N. Kondo. 1994. One subspecies of the red jungle fowl (gal/us gal/us gal/us) suffices as the matriarchic ancestor of all domestic breeds. Proc. Natl. acad. Sci. USA (91): 12505-12509

Garcia-R,J.C., Gibb,G.C. and Trewick,S.A. 2014. Eocene diversification of crown group rails (aves: gruiformes:rallidae) PLoS ONE 9 (10), E109635 (2014)PUBMED 25291147

Hajibabaei M et al. 2006. A minimalist barcode can identify a specimen whose DNA is degraded. J CompilationBlackwell Publishing. 6: 959-964

Herbert PDN, Ratnasingham S, de Waard JR, (2003) Barcoding animal life: cytochrome c oxidase subunit I divergences among closely related species. Proc R Soc Lond B 270;S96-S99

Hershkovitz, M.A. and D.D. Leipe. 1998. Phylogenetic analysis. In Baxevanis, A.D. and B.F. Oullette (Eds.). Bioinformatics a Practical Guide to The Analysis of Genes and Proteins. John Wiley and Sons, New York.

Hillis, D.M., C. Moritz, and B.K. Mable. 1996. Molecular Systematic. 2nd Ed. Sinauer Assocites, Massachusetts.

Hollingsworth PM, Forrest LL, Spouge JL, Hajibabaei M, Ratnasingham R (2009) A DNA barcode for land plants. Proc. Natl. Acad. Sci. USA. 106: 12794-12797

Kerr KCR, Stoeckle MY, Dove CJ, Weight LA, Francis CM, Hebert PDN Comprehensive DNA barcode coverage of north American birds. Mol Ecol Notes 7:754-755

Koyong,S.S, Wungouw,H.R.Lambey,L. 2014. Morfometri Burung Weris Gallirallus philippensis dan Burung Weris Gallirallus torquatus di Kota Kotamobagu Sulawesi Utara

Mardiana R. 2002. Pengelolaan dan Pengamatan Tingkah Laku Harian Burung Maleo( Macrocephalon maleo) di Taman Burung TMII. Jurusan Biologi Fakultas MIPA. IPB Bogor

Nasution, A.A. 1996. Usaha Penangkaran Burung Langka. Skripsi sarjana Jurusan Biologi. IPB Bogor

Orzech K. 2005. Sample Ethogram. 25 Februari 2009)

Quinn, T.W. and AC. Wilson. 1993. Sequence evolution in and around the mitochondrial control region in birds. J Mol. Evol (37):412-425

Reece RJ. 2004. Analysis of Genes and Genomes. Manchester: Manchester University Press.

Saitoh,T., Sugita,N., Someya,S., Iwami,Y., Kobayashi,S.,Kamigaichi,H. 2014. DNA barcoding reveals 24 distinct lineages as cryptic bird species candidates in and around the Japanese Archipelago. Mol Ecol Resour (2014) In press PUBMED 24835119

Suratmo,.F.G 1979. Prinsip Dasar Tingkah Laku Satwa Liar. Fakultas Kehutanan IPB. Bogor.

Ward RD, Zemlak TS, Innes BH, Last PR, Hebert PDN (2005) DNA barcoding australia’s fish species. Philos Trans R Soc Lond B 360:1847-1857

Ward RD, Holmes BH, White WT, Last PR ( 2008a) DNA Barcoding Australian chondrichtyans: result and potential uses in conversation. Mar Freshw Res 59:57-71

Yuwono T. 2006. Teori dan Aplikasi Polymerase Chain Reaction. Yogyakarta: Andi


  • Saat ini tidak ada refbacks.